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After electrophoresis the SDS was removed by washing the gel twice for 15 min in 2.5% (v/v) Triton X-100 followed by two further 15-min washes in protease assay buffer (50 mM Tris, 5 mM CaCl 2 , 100 M ZnCl 2 , pH 7.6)

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It was squishy, like a Slip and Slide [waterslide].[188] According to experts on pesticide exposure, appropriate personal protective equipment to limit pesticide exposure for farmworkers in most fieldwork situations is work clothing that covers the head, body, arms, legs, and feet

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And, throughout the movie, this disease causes Arthur to suffer only when he does not want to, but after the Joker's awakening, he never once laughs against his will